Automated Alkaline Phosphatase Determination
نویسندگان
چکیده
منابع مشابه
Automated determination of acid phosphatase.
A procedureis presentedfor the automated determination of acid phosphatase activity in biological materials from either plant or animal origin. The Technicon N-i flow diagram has been simplified, and the reagents for the measurement of enzymatically produced phenolic substanceshave been modified without lossof range or sensitivity. Both phenyiphosphateand a-naphthylphosphate, introduced by Babs...
متن کاملAn automated alkaline phosphatase assay with phenolphthalein monophosphate as substrate.
The manual procedure of Babson et al. (1) for the determination of serum alkaline phosphatase has been automated. The assay is based on the colorimetric determination of phenolphthalein formed on hydrolysis of phenolphthalein monophosphate. The procedure utilizes 0.16 ml. of serum without dialysis. Blanks are required only for turbid sera. Results are compared with those obtained by the manual ...
متن کاملKinetic determination of granulocyte alkaline phosphatase by the GEMSAEC.
Granulocyte alkaline phosphatase (ALP) activity is measured kinetically using the GEMSAEC centrifugal analyzer and p-nitrophenylphosphate as substrate. Measurements of enzyme activity are made at pH 9.8 utilizing 1.0 M diethanolamine as a buffer containing 0.5 mM magnesium chloride. Granulocytes are separated from heparinized blood using dextran sedimentation, followed with ficoll-hypaque separ...
متن کاملInterfering effect of bilirubin on the determination of alkaline phosphatase.
OBJECTIVE This study was designed to evaluate whether the high concentration of bilirubin is able to interfere the determination of alkaline phosphatase (ALP). METHODS Clinical tests evaluating the interfering substance of bilirubin of various concentrations on the determination of ALP were conducted based upon the Clinical and Laboratory Standards Institute (CLSI) document EP7-A2, the most r...
متن کاملA Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination
A chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chrono...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1967
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/13.4.290